Summary. This protocol describes three Drosophila preparations: 1) adult brain dissection, 2) adult retina dissection and 3) developing eye disc- brain complexes dissection. Emphasis is laid on special preparation techniques and conditions for live imaging, although all preparations can be used for fixed tissue immunohistochemistry.Cited by: 32.
Jun 04, 2008 · The LNv as a model for CNS axonal injury in Drosophila. As a first step in the development of a model for CNS axonal tract injury in Drosophila, we identified a subpopulation of neurons in the adult brain that 1) forms stereotypic axonal tracts, 2) is easy to reach i.e. superficial in the brain and 3) can be genetically manipulated. The LNv fit Cited by: 81.
FLYLIGHT(PROTOCOL(–(DISSECTION(AND(1.2%(PFA(FIXATIONFOR(ADULT(DROSOPHILA(CNS((((20150304’ ’ p.’1’ For-videos-of-dissectionof-adult-brains-see-Adult.
Mar 15, 2010 · The Drosophila brain and visual system are widely utilized model systems to study neuronal development, function and degeneration. Here we show three preparations of the brain and visual system that cover the range from the developing eye disc-brain complex in the developing pupae to individual eye and brain dissection from adult flies.Cited by: 32.